How to Become a Western Blot Expert – An All-In-One Guide to Western Blot from Start to Finish

Western blotting WB (immunoblotting) is a widely practiced analytical technique to detect target proteins within samples using antigen-specific antibodies. WB involves two major processes: Separation of soluble proteins into discrete bands and transfer of those proteins onto a solid matrix for subsequent analysis by immunological probes. In this handbook, learn about the principle of Western blotting, recommended protocols, and a troubleshooting guide to help you get the most out of your assays.