How to Become an ELISA Expert – An All-In-One Guide to ELISA from Start to Finish

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measurable product. In this handbook, learn about the different types of ELISA, methods of sample preparation, and a troubleshooting guide to help you get the most out of your assays.