How to extract RNA from hard-to-lyse samples in TRIzol

RNA isolation is a challenging procedure. Unlike DNA, which is highly stable, RNA is very unstable, quickly digested by RNase enzymes if their activity isn’t immediately inhibited. One popular reagent in RNA isolation protocols is TRIzol^®. TRIzol was specifically formulated to isolate total RNA from any sample type in the modern research laboratory, whether in academia or industry: cells, hard to lyse or solid tissue, and infectious biological samples. In this application, Zymo Research present the best RNA extraction methods for challenging sample types, such as hard-to-lyse samples.