How to influence oligonucleotide analysis by IP-RP

In this application note, YMC presents experimental data on oligonucleotide analysis using the YMC-Accura Triart Bio C18 column. The purity and quality of oligonucleotides needs to be controlled closely since impurities can affect their efficacy. The analysis of oligonucleotides is highly challenging due to their negatively charged phosphate groups in the backbone. Ion pair reversed phase liquid chromatography (IP-RP) is considered the gold standard for oligonucleotide analysis.