How to isolate and count intact nuclei

This eBook explores advances in quality control methods for nuclei sample prep

6 Nov 2024

Isolating clean, intact nuclei is vital for the success of single-nucleus workflows such as RNA sequencing (RNA-seq) and Assay for Transposase-Accessible Chromatin with sequencing (ATAC-seq). High-quality, debris-free samples are essential for accurate library preparation and downstream analyses, as debris can hinder precise nuclei counting. Traditional manual counting methods, while reliable, are time-consuming and prone to human error, especially with large sample volumes.

This eBook explores automated nuclei counting and quality control using the DeNovix CellDrop Automated Cell Counter. With features like DirectPipettetechnology that eliminates costly consumables, advanced fluorescence applications, and user-friendly software, researchers can improve accuracy, efficiency, and consistency in sample preparation. Discover practical tips to streamline workflows, minimize variability, and enhance experimental outcomes.

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