hTERT-immortalized and Primary Keratinocytes Differentiate into Epidermal Structures in 3D Organotypic Culture

Primary and immortalized keratinocyte cultures are essential models for investigating the normal and pathological biology of the epidermis. This application notes demonstrates how an immortalized keratinocyte cell line, Ker-CT, is able to differentiate into organotypic skin equivalents in a 3D culture model.