HTRF cAMP HiRange Assay on SpectraMax Multi-Mode Microplate Readers from Molecular Devices

The HTRF cAMP HiRange kit enables quantitation of cyclic AMP (cAMP, cyclic adenosine 3’, 5’–monophosphate) in cellular samples. In this application note we show how the SpectraMax® i3, SpectraMax® Paradigm®, and SpectraMax® M5e Multi-Mode Microplate Readers are used to perform robust, high-throughput HTRF® assays with excellent Z’ factors and highly reproducible EC50 values. The readers are streamlined and stable for HTS and time to results is faster with SoftMax Pro Software protocols.