HTRF Human TNFα Assay on SpectraMax Paradigm Multi-Mode Microplate Reader

HTRF® is a versatile technology developed by Cisbio Bioassays for detecting biomolecular interactions. It combines fluorescence resonance energy transfer (FRET) technology with time-resolved (TR) measurement of fluorescence, allowing elimination of short-lived background fluorescence. This application note shows how the SpectraMax® Paradigm® Multi-Mode Microplate Reader is used to perform robust, no-wash cytokine assays with excellent Z’ factors, using the HTRF TNFα assay.