Human Embryonic Stem Cells Grow on Feeder-Free and Chemically Modified Plastic Plates

In this application note hESC TW1 are cultured on a chemically modified plastic plate (Corning® Synthemax Surface) with mTeSR medium (a serum-free medium, StemCell Technologies, Inc.) to establish a new feeder-free culture system. Alkaline phosphatase (AP) staining, immunofluorescence staining and flow cytometry is used to analyse hESC TW1 cells for AP activity, typical pluripotent markers and the ability to differentiate into the three germ layers in vitro.