Human PBMC Isolation and Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter

The Scepter™ cell counter combines the ease of automated instrumentation and the accuracy of impedance-based particle detection using the Coulter principle in a handheld format. This application note outlines a protocol for the isolation of PBMC from whole blood and subsequent sample analysis using the Scepter™ cell counter.