Hydrophobic interaction and reversed phase chromatography

Hydrophobic interaction chromatography (HIC) separates biomolecules in relatively mild conditions according to differences in their hydrophobicity. Many scientists use HIC in protein purification as a complement to other techniques that separate according to charge, size, or biospecific recognition.

Explore both theoretical and practical aspects of the technique, the resins available, and how to select them, together with application examples, and detailed instructions for the most common procedures.