iCell Endothelial Cells: Assaying Cell Proliferation

Regulation of endothelial cell proliferation plays a fundamental role in vascular remodeling and angiogenesis in several physiological and pathological conditions. This application note describes the use of iCell® Endothelial Cells, from Cellular Dynamics International, in proliferation assays. iCell Endothelial Cells were treated with a growth factor and/or its receptor inhibitor to modulate a known pro-angiogenic signaling pathway. The proliferation activity was reliably assessed using the CellTiter-Glo® Luminescent Cell Viability Assay, from Promega.