iCell® Cardiomyocytes – Immunofluorescent Labeling Application Protocol

Immunofluorescent labeling is a straight-forward technique for assessing the presence and the subcellular localization of antigens and/or proteins. Several labeling methods are available depending on the biological sample, cell preparation, and availability of antibodies against the target. The protocol presented here has demonstrated utility in labeling cardiac troponin T, myosin light chain, sarcomeric alpha-actinin, connexin-43, and N-cadherin in iCell® Cardiomyocytes. This protocol should serve as a guide for immunofluorescent labeling other cardiac proteins.