ICPL Labeling in Functional Proteomics Experiments: Substrate Identification of the Extracellular Protease ADAMTS1 using SDS-PAGE LC-MS/MS

This application note demonstrates an Isotope-Coded Protein Label (ICPL [1,2]) investigation in a workflow using SDS-PAGE for protein pre-fractionation, a Bruker Ion Trap mass spectrometer, and ProteinScape for quantitative analysis.