Identification of B Cells, NK Cells, Multiple T Cell Subsets and Myeloid Cells by 13-Parameter Immunophenotyping of Human Lysed Whole Blood

Immunophenotyping is the analysis of heterogeneous populations of cells within human blood for the purpose of identifying the presence and proportions of various cell populations. This application note describes the use of the Attune NxT Flow Cytometer with 4 lasers and 13-color immunophenotyping analysis of stained human whole blood using a stain/lyse protocol. Lymphocyte, monocyte and granulocyte populations were distinguished with forward scatter (FSC) and side scatter (SSC); and monocyte, T cell, B cell and NK populations were identified using fluorescent antibodies against surface antigens specific for the different immunological populations.