Identification, Quanification and Annotation of Heart Membrane Proteins Using Label-Free Nanoscale LC /MS

Heart diseases resulting in heart failure are among the leading causes of morbidity and mortality in the Western world and can result from either systemic disease (e.g. hypertensive heart disease, ischaemic heart disease) or specific heart muscle disease (e.g. dilated cardiomyopathy (DCM)). Sub-proteome analysis of such disease sub-sets affords a reduction in sample complexity, potentially revealing biomarkers of cardiac failure that would otherwise remain undiscovered. Label-free nanoscale LC/MS is used in this study to validate a Triton X-114 based enrichment method for cardiac membrane proteins.