Illicit Drug Analysis in Urine Using 2D LC-MS/MS for Forensic Toxicology

The use of spot testing is the most known technique in forensic analysis for rapid results. For a complete legal identification, a more robust methodology is required, often LC-MS or LC-MS/MS. However, to achieve satisfactory results most often with trace level detection requirements, extensive and time consuming sample preparation protocols and required to reach sub ppb levels. This application note demonstrates the use of multi-dimensional chromatography as a micro extraction technique and sequential 2D extraction for the analysis.