Imaging and Analysis of a High Density Cell Migration Assay

The migration of cells commonly occurs during both normal biological processes such as the development of an organism or marking pathological events such as cancer metastasis. This study evaluated the use of a high density cell exclusion assay in 384-well format for investigation of cell migration using a cell line stably expressing green fluorescent protein (GFP). In this study, images were captured by the Cytation™ 5 and analyzed using automated digital wide field fluorescence microscopy.