Imaging of BacMam Transfected U-2 OS Cells: Optimization of Transfection Conditions Using the Cytation™3 Cell Imaging Multi-Mode Reader and Gen5™ Data Analysis Software

Transient transfection of tissue culture cells is a routine method to introduce novel genetic elements into cultured cells. While there are numerous methods to introduce foreign DNA into tissue culture cells the efficacy of the experimental results is dependent on adequate expression of the introduce gene. This application note describes the use of the Cytation™ 3 Cell Imaging Multi-Mode Reader to optimize and assess gene expression and transfection efficiency in live cells after transfection using a BacMam gene delivery system.