Immunofluorescence Visualization of Fibroblast or Cancer Cells in RAFT™ 3D Cell Cultures Technical Notes

This technical note demonstrates that immunofluorescence can be performed easily and routinely on RAFT™ 3D Cell Cultures. Primary human dermal fibroblasts (HDF) or the breast cancer cell line MCF7 were cultured in RAFT™ 3D culture system for up to 23 days prior to fixation and staining. This application note provides two protocols, which differ slightly depending on whether the cells maintain themselves singly within the culture or whether, as in the case of a number of cancer cell lines, they come together to form higher order structures or aggregates which we have termed tumoroids.