ResourceLife Sciences
Immunoprecipitation of IL-6 from human serum for next-generation protein sequencing on Platinum
23 Jan 2024In this application note, the focus is on enriching low-abundant proteins from complex biological matrices in proteomic workflows. The technical note introduces an immunoprecipitation protocol compatible with Quantum-Si's next-generation protein sequencing workflow. This protocol involves an on-bead digestion approach, eliminating the sample elution step and proceeding directly to the preparation of digested peptide libraries. The feasibility of this method is demonstrated through the specific enrichment of IL-6 via immunoprecipitation from human serum, followed by protein sequencing and identification on Quantum-Si's Platinum® Next-Generation Protein Sequencer™.