Improved Analytical Method for the LC/MS/MS Analysis of Chloramphenicol in Shrimp and Other Marine Food Products using Strata™-X Solid Phase Extraction Cartridges and Kinetex® 2.6 μm Core-Shell Columns

This application note from Phenomenex Inc. describes a method for the analysis of Chloramphenicol in shrimp which has been developed with a limit of quantitation (LOQ) of 0.001 ng/g in shrimp (0.001 ppb) based on the calibration standards. This is three hundred times lower than the current United States Food and Drug Administration (USFDA) method. The method described uses Strata-X solid phase extraction (SPE) cartridges for sample cleanup and concentration, followed by ultra-fast LC/MS/MS analysis (<5 minutes) using a Kinetex core-shell column.