Improved confirmation of STEC contaminants using the Thermo Scientific SureTect E. coli O157:H7 and STEC Screening and Identification PCR Assay

Microflora from food samples pose a challenge for shiga-toxin producing Escherichia coli (STEC) culture confirmation testing. In this poster, the performance of the Thermo Scientific™ SureTect™ E. coli O157:H7 and STEC Screening and Identification PCR Workflow is assessed. The SureTect confirmation plating method using Chromogenic Coliform Agar (CCA) is shown to enable highly contaminated STEC samples to be confirmed rapidly and reliably.