Improved Imaging of Cleared Samples with ZEISS Lightsheet Z.1: Refractive Index on Demand

This application note demonstrates a novel technique to formulate the optimum refractive index (RI) without compromising image quality or damaging the solvent-vulnerable Light Sheet Fluorescence Microscopy (LSFM) system. LSFM uses a thin plane of light (light sheet) to optically section transparent tissues or whole organisms that have been fluorescently labeled. This “RI matching” solution is based on the published 3DISCO, an organic-solvent based protocol, which requires no special equipment to provide a faster imaging solution compared to laser scanning microscopes.