Improving a High Sensitivity Assay for the Quantification of Teriparatide in Human Plasma Using the ionKey/MS System

This study combines μElution SPE and the novel and highly efficient ionKey/MS system to improve a quantitative assay for teriparatide in human plasma. Teriparatide is a recombinant form of a fragment of human parathyroid hormone, used in the treatment of osteoporosis. Peptides in general are often difficult to analyze by LC-MS/MS, as MS sensitivity is low due to the formation of multiple precursors and poor or overly extensive fragmentation. IonKey/MS can provide increased sensitivity compared to 2.1 mm I.D. chromatography with the same injection volume, or equivalent or greater sensitivity with reduced sample consumption, making it ideal for peptide analyses.