ResourceLife Sciences
Improving Data Quality and Turnaround Times for Targeted Next-Generation Sequencing of FFPE DNA using a KAPA HyperPlus/SeqCap EZ workflow
24 Mar 2016This application note evaluates the KAPA HyperPlus Kit from Kapa Biosystems for enzymatic DNA fragmentation and library construction. Formalin-fixed paraffin-embedded (FFPE) tissue is an important source of DNA for cancer genomic studies and clinical diagnostics. The KAPA HyperPlus Kit with integrated enzymatic fragmentation streamlines the SeqCapTM EZ Target Enrichment workflow, yielding high quality libraries.
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Sample PreparationGenome AnalysisHigh-Throughput ScreeningPCR and Thermal CyclingAssay AssemblyDNA / RNA Extraction and PurificationDNA SequencingGene Expression and Molecular CloningBiomarkersNext Generation SequencingLibrary GenerationTarget-EnrichmentHigh ThroughputLibrary PreparationDNA Amplification