Improving Effectiveness in Method Development by Using a Systematic Screening Protocol

In this application, Waters present the development of a UPLC method for metoclopramide HCl and related substances. UV (PDA) and mass detection is combined, with the user-friendly ACQUITY QDa Detector. A systematic protocol is employed that includes scouting, screening, and optimization steps. Results for each step are analyzed and ranked using custom calculations and reported within Empower 3 Chromatography Data Software to minimize analyst bias in decision making and ensure the overall goals are achieved.