Improving Intact IgG Separations with Aeris™ WIDEPORE Core-Shell HPLC/UHPLC Columns

A widepore core-shell HPLC/UHPLC column (Aeris WIDEPORE) has been introduced that is specifically designed to improve protein separations. Rather than utilize a similar morphology of small molecule core-shell columns with larger pores, a completely different particle was developed that takes into account the slower diffusion of proteins into porous particles. When one looks at different intact protein separations, the resolution of different glycoforms of therapeutic IgG antibodies probably stands out as one of the more difficult due to the large size and structure of IgG. In this article improved separation of IgG is shown using Aeris coreshell HPLC/UHPLC columns.