Improving Reliability and Reproducibility of Real-Time PCR

Clear adhesive sealers are a convenient sealing option for realtime quantitative PCR because they transmit light effectively and are easy to use. When choosing a sealer, however, it is important to consider its sealing strength as well as its clarity. Many adhesive sealers lack the aggressive adhesion necessary for properly sealing a PCR plate, and therefore can produce inconsistent results. The results of this study demonstrate that effective sealing is
important for achieving low variability and a high success rate in quantitative PCR.