Improving the Productivity in Isolating a Naturally Occurring Bioactive Compound Using Supercritical Fluid Extraction and Preparative Supercritical Fluid Chromatography

This application demonstrates the use of SFE and prep SFC to holistically improve the productivity in isolating a low-polarity, bioactive compound from a complex natural product extract. The SFE alleviated the sample complexity prior to analysis and purification, thereby improving sample loading and reducing solvent use in the ensuing chromatography. The SFE extract also lends itself well for SFC analysis and purification.