In-Depth Identification of Protein Images by Combining High Mass Resolution MALDI FTICR Imaging and High Performance qTOF nLC-MS/MS

ImageID is a workflow for improving the number of proteins identified from a MALDI imaging dataset. Typically pairing LC-MALDI and MALDI TOF imaging, ~ 100 proteins have been identified and imaged from rat brain sections. The study described in this poster examines if the number of proteins identified and consequently imaged can be increased by modifying the imageID workflow to utilize high resolution MALDI-FTICR imaging with high-performance qTOF LC-MS/MS.