In-House Sulfur-SAD Phasing of Thaumatin from Thaumatococcus danielii

The crystal structure of Thaumatin from Thaumatococcus danielii was determined by sulfur-SAD phasing methods using data collected on a D8 VENTURE diffraction system. The D8 VENTURE features a new CMOS detector, the PHOTON 100. Using one crystal, a complete data set was collected to 1.65 Å in less than 3 hours. The experimental phases derived from the anomalous signal of the sulfur atoms allowed 95% of the protein backbone to be traced.