In Vitro Diagnostics: CEDIA Cyclosporine PLUS Assay

The Thermo Scientific™ CEDIA® Cyclosporine PLUS assay is for the in vitro quantitative determination of cyclosporine in human whole blood using automated clinical chemistry analyzers as an aid in the management of cyclosporine therapy in kidney, liver, and heart transplants. The assay uses recombinant DNA technology to produce a unique homogeneous enzyme immunoassay system. The assay is based on the bacterial enzyme ß-galactosidase, which has been genetically engineered into two inactive fragments. These fragments spontaneously re-associate to form fully-active enzymes that, in the assay format, cleave a substrate, generating a color change that can be measured spectrophotometrically.