Incorporation of a Novel 3D Cell Culture System to Perform <em>in vitro</em> Cytotoxicity Analyses using Human Primary Hepatocytes

Cytotoxicity, and in particular hepatotoxicity studies, are a common part of drug discovery, as well as other industries such as the cosmetics industry. The ability to perform long-term (multiple week) studies to determine the effect of chronic dosing of a test molecule is critical to ascertain proper data before going to clinical studies. Hepatocytes cultured in 3D give researchers the ability to accomplish this which did not exist when using 2D culturing techniques. This application note helps to further demonstrate how the Cytation 3 can be used with cells cultured in 3D.