Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC Using Unique Biphenyl Column Selectivity

The analysis of antibiotic residues in food-producing animals is important worldwide for evaluating food safety and maintaining compliance with export regulations. Sulfonamides are a specific concern, as drugs in this antibiotic class are commonly used in feed additives for livestock in order to fight infections and maintain desired growth levels. In this application note, the unique selectivity and high retention of biphenyl columns produce complete separations and benefit both UV and MS detection. In addition, biphenyl columns in a UHPLC format allow faster sample throughput, while maintaining good separation of target compounds.