Increase Sensitivity in No-Wash Assays Using Confocal Imaging

Screening of hybridoma supernatants for antibodies directed against cell-surface antigens is an important step in the discovery and development of antibodies for vaccines or therapeutic use. The sensitivity of these assays is increased by taking advantage of the optical properties of confocal imaging such as utilizing the narrow depth of field to acquire high signal-to-background, crisp images with no out of focus light creating image blur.