Increased Efficiency of Biomolecule Identification by Optimization of Trypsin Digestion Buffers

Protein insolubility can be a major impediment to traditional bottom-up proteomic methods because it reduces the efficiency of enzymatic digestions and makes analysis problematic. Insolubility can be caused by a variety of factors, including the salt concentration, the presence of various organic solvents, temperature, or simply the nature of the protein itself. This application note demonstrates how the Thermo Scientific™ SMART Digest™ kit removes the uncertainty associated with conventional solution-based tryptic digestion protocols, resulting in higher efficiency and sample characterization.