Increased Protein and Proteome Coverage Using Informatics-Based Gas-Phase Fractionation

In this application note Progenesis LC-MS is used to complement informatics-based gas-phase fractionation (iGPF) to increase protein/proteome coverage when using replicate analysis of label-free samples. When iGPF was applied to quantitative proteomic analysis of Schizosaccharomyces pombe the number of proteins identified was doubled compared to when no GPF was used.