Increased Viability and Genomic Integrity of CRISPR-Modified hiPS Cells Selected with WOLF Cell Sorter Microfluidic Technology

Human iPS cells (hiPSC) can be modified utilizing CRISPR/Cas9 gene editing technology to generate in vitro models of human diseases. Critical shortcomings in the generation of CRISPR-modified clonal lines are: 1) low viability/proliferation, and 2) genomic abnormalities following selection via fluorescent activated cell sorting. To address these issues, a microfluidic cell sorter (WOLF^® Cell Sorter, NanoCellect) is compared to a droplet cell sorter in Alzheimer’s disease (AD) and autism spectrum disorder (ASD) model cell lines.