Increasing amplicon spots detection efficiency

The field of spatial transcriptomics aims to map the RNA transcriptome of single cells within tissues while preserving spatial information. CrestOptics addresses challenges faced in imaging-based spatial biology, particularly optical crowding when using epifluorescence microscopy. A study is presented that explores the impact of structured illumination microscopy (SIM), specifically the DeepSIM super-resolution (SR) system, on single-gene transcript detection. CrestOptics demonstrates that DeepSIM enhances RNA spot detection compared to widefield (WF) and confocal (CF) modes, improving contrast, resolution, and spot detection performance.