Induction and Inhibition Studies of Hypoxia and Oxidative Stress in Immortalized Keratinocytes

This application note describes a multiplexed assay that can monitor the induction of hypoxia and oxidative stress phenotypes using fluorogenic probes. Data readout can be achieved using whole well fluorescence intensities measured using either monochromators or spectral filters for rapid measurements depicting relative phenotype changes. Absolute ratios of effected cells for each phenotype can also be obtained through cellular analysis using automated digital widefield fluorescence microscopy. All cell-based assays were performed using the Cytation™3 Cell Imaging Multi-Mode Reader.