Infrared Detection Decreases Nitrocellulose Autofluorescence and Improves RPPA Assays Signal‐to‐Noise Ratio Over Visible Wavelength Detection

Reverse Phase Protein Arrays (RPPAs) are a specific type of protein arrays consisting of printed cell lysates useful for cell signaling and biomarker analysis. RPPAs are mainly built on nitrocellulose film slides as they have high binding capacity and viability for native proteins. Until now, RPPA assays with fluorescence detection were limited in sensitivity by high intrinsic background fluorescence normally produced by the nitrocellulose; in this application note learn how it is now possible to increase the Signal to Noise Ratio using Near Infrared (NIR) detection with the InnoScan 710‐IR scanner.