Intact identification of adeno-associated virus capsid proteins

In this application note, Phenomenex profiles denatured and dissociated capsid proteins from AAV5 and AAV9, via analysis with LC-MS using a BioZen^™ Intact XB-C8 reversed phase column. The use of adeno-associated viruses (AAV) as an effective vector for the delivery of ssDNA in gene therapy continues to gain momentum. A chief attribute to this success is the broad tissue tropism enabled by a range of serotypes. To date, approximately 13 different serotypes have been identified with a range of shared amino acid sequence identity. Structurally, the viral capsid is a sixty-protein icosahedron comprised of three capsid, or viral proteins (VP), non-covalently assembled in a ratio of 1:1:10 (VP1:VP2:VP3).