Intravital Imaging of a Mouse Ventral Skin-Flap Using the iBox® Explorer™ Microscope

Critical to the investigation of intravital migration imaging is the use of fluorescent proteins. In this application note, nude athymic mice were injected with GFP/RFP-labeled human fibrosarcoma cells. The migration of cells within the venous circulation was then investigated using the iBox Explorer Imaging Microscope (UVP, LLC). Using an optical train that allows for imaging from the whole organ to cellular level (2.24 pixels/micron resolution), cancer cells were visualized migrating within the systemic circulation.