Intrinsic tryptophan detection with the SpectraMax i3 Multi-Mode Microplate

This applications note demonstrates performance of the SpectraMax® i3 Multi-Mode Microplate Platform for assays measuring intrinsic tryptophan fluorescence. High sensitivity is shown with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. Lysozyme contains two tryptophan residues that fluoresce when excited with UV light. When lysozyme is denatured, tryptophan's emission peak typically shifts about 6-10 nm. This peak shift can be measured by performing fluorescence spectral scans on native and denatured lysozyme.