IRDye® Peptide Labeling with Odyssey® Imaging Systems

Peptides labeled with fluorescent dyes are important as probes for in vivo imaging and as substrates for enzyme activity assays. Near-infrared (NIR) fluorophores such as IRDye® 800CW (excitation 780 nm; emission 794 nm) can offer improved sensitivity because of low NIR autofluorescence from tissues, cells, biological materials, or drug compounds. Fluorophores can also be combined with appropriate quencher dyes to create fluorogenic peptide probes, such as those used for protease activity detection. This application note provides an overview of IRDye® Peptide Labeling with LI-COR’s Odyssey® Classic, Odyssey CLx, Odyssey Sa, and Aerius Imaging Systems.