Isolation and Characterization of Analytes in Serum

The high concentration of phospholipids in blood serum is a frequent limitation in metabolomics analysis based on mass spectrometric detection, mainly owing to their ionization suppression effects. Using HybridSPE®- Phospholipid solid phase extraction followed by LC -MS is useful for global metabolome analysis by fractionation of phospholipid and non-phospholipid analytes.