Isolation of Total RNA from a Human Cell Line (HEK 293) Using TRIzol® in Eppendorf Tubes® 5.0 mL

Since RNA isolation using TRIzol is freely scalable, sample volumes may fall between 2 and 5 mL, depending on the starting material. Such preparations cannot be performed in the classic microcentrifuge tubes up to 2 mL. This application note describes the isolation of RNA using TRIzol, with the aim of comparing the Eppendorf Tube 5.0 mL to a standard conical 15 mL vessel with regards to RNA yield and handling.