Isolation of Viral RNA, Viral DNA and Bacterial DNA from Animal Samples

Successful pathogen identification employing real-time PCR or RT-PCR is dependent on a multitude of parameters of which the isolation of high-quality nucleic acid is paramount. However, increasing the overall efficiency, and thus success rate, of veterinary pathogen detection can also be achieved by parallel and automated processing of different sample types. In this article, we describe how this can be achieved. Utilizing a combination of the QIAamp^® cador^® Pathogen Mini Kit, the QIAgility^® for automated PCR setup, and universal real-time PCR cycling protocols with the QuantiFast^® Pathogen PCR and RT-PCR +IC Kits, on the Rotor-Gene^® Q, permits multi-pathogen identification from multiple sample types.