ISSR-PCR Fingerprinting of Plant Pathogen Strains using the QIAxcel® System and BioNumerics®

ISSR stands for Inter Simple Sequence Repeat and refers to the genome regions between microsatellite loci. It is a rapid and inexpensive genotyping technique with a wide range of applications, including the characterization of genetic relatedness among the organisms of a population. This application describes the amplification of ISSR sequences from individual C. michiganensis subsp. michiganensis strains using ISSR-PCR and the QIAxcel Advanced System.